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50例血小板减少标本结果分析

时间:2017-09-13 22:00:05来源:www.xielunwen.net 作者:admin 点击: 30 次
作者:章小东,彭兰芬,黄文彩,尹艳丹,余小丹 【关键词】CD [摘要]目的:探讨CD1700血细胞分析仪血小板(PLT)检测结果偏低的原因,选择正确的方法进行复查,保证PLT检测质量。方法:对50例CD1700血细胞分析仪上检测结果偏低的标本涂片镜检,重新采集静脉血,分别在CD1700(预稀释法)上和用手工法进行复查。对血涂片存在PLT聚集的抗凝血在首次检测30min后在CD1700上再复

                     作者:章小东,彭兰芬,黄文彩,尹艳丹,余小丹

【关键词】  CD

  [摘要] 目的:探讨CD1700血细胞分析仪血小板(PLT)检测结果偏低的原因,选择正确的方法进行复查,保证PLT检测质量。方法:对50例CD1700血细胞分析仪上检测结果偏低的标本涂片镜检,重新采集静脉血,分别在CD1700(预稀释法)上和用手工法进行复查。对血涂片存在PLT聚集的抗凝血在首次检测30 min后在CD1700上再复查一次。结果:有40例标本血涂片PLT形态正常,无PLT聚集,重新采血复查PLT,两种方法结果仍偏低,与复查前差异无显著性(P>0.05)。10例标本血涂片上PLT存在着不同程度的聚集,重新采血复查PLT,两种方法结果均正常,与复查前差异有显著性(P<0.01) 。10例标本中有2例在CD1700上复查结果正常。结论:对各种原因引起PLT检测结果偏低者,应进行人工镜检血涂片和PLT手工计数,或在其他仪器上检测,同时要提高采集血涂标本的质量。

  [关键词] CD1700血细胞分析仪;血小板聚集;复查

  Results Analyse to 50 Cases of Thrombocytopenia Specimens

  Abstract:Objective To explore the reasons that result in platelets analysis results on low side which analysed in haematology analyzer CD1700 in order to select correct methods to reexamine them and then guarantee the quality of platelet analysises.Methods 50 Cases of thrombocytopenia speciments analysed in haematology analyzer CD1700 were made into bolld smears and examined in microscope,and the suffers of these speciments were collected blood again from vein 50 samples of blood were analysed separately in haematology analyzer CD1700 (prediluted method) and with manual method.The speciments which with platelet aggragation in blood smears were analysed in CD-1700 30 minutes after first analysed.Results In 40 Cases of speciments the shapes of platelets was normal and there were not the low side and were not different from which analysed first time(P>0.05).For 10 Cases of speciments with plateiet aggregation of differents degrees in blood smears the platelet results reexamined with two methods were different from the results analysed fist time(P<0.01).And two speciment of the ten was analysed again 30 minutes afer fist analysed,the result was normal.Conclusion Thrombocytopenia results caused by kins of reasons should be analysed by examining blood smears in microscope,and pletlets were analysed with manual method or in other haematology analyzer,simultane the quality of collecting blood speciments should be improved.

  Key words:Haematology analyzer CD1700;Pletlet/ aggregation;Reexamine

  随着全自动血液分析仪日益普及和使用,大大提高了血常规检测结果的精确性和准确性,用CD1700血细胞分析仪进行血细胞分析时常遇到血小板(PLT)直方图异常且PLT数目减少的样本,PLT直方图上偶尔出现“LYM RO”或“RM”提示,笔者就50例PLT数量减少的病例进行预稀释计数、手工计数,报道如下。

  1 材料与方法

  1.1 仪器 试剂CD1700血细胞分析仪(美国雅倍) 及配套质控、定标试剂,仪器均经校正。

  1.2 PLT稀释液(许汝和氏法) 用于显微镜计数(手工法),按《全国临床检验操作规程(第二版)》配制。

  1.3 EDTAK2真空抗凝管 湖北金杏专用EDTAK2凝血管。

  1.4 实验方法 对50例CD1700血细胞分折仪上检测结果偏低的标本涂片镜检,并对患者重新采集静脉血,分别在CD1700(预稀释法)上和用手工法进行复查。两种方法复查结果正常者30分种后用抗凝血在CD1700上再复查一次。

  1.5 检测结果 作配对t检验。

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