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夹竹桃对蛙心收缩力的影响及其机制探讨

时间:2017-12-14 09:00:04来源:www.xielunwen.net 作者:admin 点击: 1 次
【摘要】目的探讨不同剂量的夹竹桃和毒K(毒毛花苷(甙)K)对蟾蜍心脏的作用及其相关机制。方法采用离体蟾蜍心脏,用含戊巴比妥钠(2mmol/L,1ml)的任氏液造成蟾蜍心衰模型(心肌收缩力降低一半时为心衰),经张力换能器、BL-410智能生物信号显示与处理系统记录心肌的收缩活动的曲线。5min作为对照组,然后观察药物对其作用,2min后曲线变化,并与对照组比较。结果灌流
【摘要】  目的  探讨不同剂量的夹竹桃和毒K(毒毛花苷(甙)K)对蟾蜍心脏的作用及其相关机制。方法 采用离体蟾蜍心脏,用含戊巴比妥钠(2mmol/L,1ml)的任氏液造成蟾蜍心衰模型(心肌收缩力降低一半时为心衰),经张力换能器、BL-410智能生物信号显示与处理系统记录心肌的收缩活动的曲线。5min作为对照组,然后观察药物对其作用,2min后曲线变化,并与对照组比较。结果 灌流不同浓度的夹竹桃液后,2%的夹竹桃液(0.2ml)使心肌在2min内收缩频率迅速加强,幅度也达到最大,10min后心肌收缩频率和幅度再缓慢减弱恢复至对照组水平。而2%(0.2ml)的毒K连续观察2min,5min,8min心肌收缩频率没有变化。结论 一定浓度的夹竹桃液对心肌的收缩力和幅度都有所加强,而同一浓度同一时间的毒K心肌收缩率和幅度呈一直线,2%的夹竹桃液增加心肌收缩率和幅度作用较强,时间较短,而毒K时间长,剂量大。二者比较差异显著。结论  2%的夹竹桃液对心肌的收缩力和幅度都有加强,而毒K治疗量接近中毒量的60%。
【关键词】  医夹竹桃  离体蟾蜍心脏  蟾蜍
【Abstract】 Objective: Finding out the impact and the relevant mechanism of different dosages of oleander and Strophanthin k on the heart of toad; Approach: Forming a heart-failure model making use of Ringer’s solution containing Sodium pentobarbital (2mmol/L, 1ml) with in vitro of the heart of toad (When the myocardial contraction force is decreased by a half, it is called heart failure); the curves of contraction is recorded with a tonotransducer and a BL-410 bio-signal display and processing system. The result after five minutes is taken as a control group and then the impact of drug on it is observed; after 2 minutes the curve is changed and the it is compared with the control group. Results: After feeding different densities of oleander, it is found that the contraction frequency of the myocardial is rapidly intensified and the amplitude is the largest after 2 minutes when 2% (2ml) density of oleander is fed; after ten minutes, the contraction frequency of the myocardial is slowly receded and finally reach the level of that of the control group. While 2% density of Strophanthin k is fed and the continuous observation is found that after 2 minutes, 5 minutes and 8 minutes the frequency of contraction of myocardial is not changed. Conclusion: certain concentration oleander will intensify the frequency and amplitude of myocardial contraction, but when the Strophanthin k of the same concentration and time is fed, the frequency and amplitude of myocardial contraction will become a straight line. 2% concentration oleander will have a stronger impact on the frequency and amplitude of myocardial contraction in a shorter time, while more dosage of Strophanthin k and more time are needed to have the same impact with that of oleander. There is a marked deference between the action of oleander and Strophanthin k. The curative dose of Strophanthin k is about 60% of those of moderately toxic.
【Key words】 Oleander  Isolated toad heart  Toad
        夹竹桃属于强心类中药。味苦、性寒、有毒,归心经。主要功能为强心利尿、祛痰定喘、镇痛、祛瘀。近代临床运用该药治疗心脏病心力衰竭、喘息咳嗽、癫痫、跌打损伤、经闭、斑秃。本品有毒,内服煎汤,剂量为0.3~0.9g。
        1 材料与方法
        1.1材料
        取夹竹桃25g,洗净用纱布擦干,剪碎,置广口瓶内,加70%乙醇100ml,室温浸泡48h,并经常振摇,用棉花过滤,再用70%乙醇适量洗叶片,挤压取滤液,滤液在水浴锅上蒸干,得粗醇浸出液,用适量任氏液溶解浸出物,用滤纸过滤,最后用任氏液加至250ml,既为10%夹竹桃任氏液置冰箱备用。(用时稀释10倍) (责任编辑:admin)
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